MEP途径

Anti-DXS / 1-deoxy-D-xylulose-5-phosphate synthase

Anti-DXS / 1-deoxy-D-xylulose-5-phosphate synthase

    货 号: PAB18001
    基 因: AT4G15560
    别 名: 1-deoxy-d-xylulose 5-phosphate synthase, ATCLA1, DXS.
    验证物种: 拟南芥
    推荐稀释比: WB 1:2500---1:5000

 

背 景

1-deoxy-D-xylulose-5-phosphate synthase catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP). It’s a limiting enzyme for plastidic isoprenoid biosynthesis and essential for chloroplast development. It’s involved in the MEP pathway. It is essential for chloroplast development in Arabidopsis

免疫原

Recombinant protein of Arabidopsis thaliana AT4G15560.

同源基因

LOC_Os05g33840, Os05g0408900, Zm00001d038170, GRMZM2G137151, GLYMA07G38260, etc.

别 名

1-deoxy-d-xylulose 5-phosphate (DXP) synthase 1, 1-deoxy-d-xylulose 5-phosphate synthase, 1-deoxy-d-xylulose 5-phosphate synthase 2, ATCLA1, CLA, CLA1, CLOROPLASTOS ALTERADOS 1, DEF, DXPS2, DXS, DXS1.

验证应用

Western Blot

物种反应性

The sequence of the recombinant protein used for immunization is homologues with the sequence in Arabidopsis thaliana, rice, maize, soybean, rape, pepper, barley, etc.

For more species homologues information, please contact tech support at TEL 400-880-3241

保存

50% glycerol,

推荐稀释比

Western Blot (1:5000)

理论 / 实际分子量

77 / 75 kDa for Arabidopsis thaliana

检测图片

 

 

上样: 1ug拟南芥/水稻叶片总蛋白

电泳:12.5% SDSPAGE

转膜: NC (nitrocellulose) 膜, 电压10 v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h