蛋白折叠、修饰、降解、转运等

Anti-BIP1/2 /Heat shock 70 kDa protein rabbit antibody

Anti-BIP1/2 /Heat shock 70 kDa protein rabbit antibody

    货 号: PAB211003
    描 述:兔多克隆抗体
    基 因: At5g42020, At5g28540, LOC_Os02g02410
    别 名:BIP, LUMINAL BINDING PROTEIN, Heat shock protein 70-12
    验证物种:拟南芥,水稻
    推荐稀释比:WB 1:2500---1:5000

 

背 景

Hsp70s are key components that facilitate folding of de novo synthesized proteins, assist translocation of precursor proteins into organelles, and are responsible for degradation of damaged protein under stress conditions (Probable). Involved in polar nuclei fusion during female gametophyte development and is essential for the regulation of endosperm nuclei proliferation.

抗原信息

Recombinant protein of Arabidopsis thaliana At5g42020

同源基因

At5g42020, At5g28540, LOC_Os02g02410

验证应用

Western Blot

预测物种

油菜、辣椒、番茄、大豆、土豆、玉米、小麦、高粱、等

保存条件

50% glycerol-20 °C

理论/ 实际分子量

74 kDa for Arabidopsis thaliana and rice

检测图片

 

 

上样:约2μg拟南芥和水稻叶片的总蛋白

电泳:12.5% SDS-PAGE

转膜:NC (nitrocellulose) 膜,电压10v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h