蛋白折叠、修饰、降解、转运等

Anti-PLSP1/ Chloroplast processing peptidase

Anti-PLSP1/ Chloroplast processing peptidase

    货 号:PAB220203
    描 述:兔多克隆抗体
    基 因:AT3G24590, LOC_Os02g16709
    别 名:Signal peptidase I-3, PLASTIDIC TYPE I SIGNAL PEPTIDASE 1
    验证物种:拟南芥,水稻
    推荐稀释比:WB 1:2500---1:5000
 
背 景
PLSP1 encodes a signal peptidase Plsp1 (plastidic type I signal peptidase 1). It is required for thylakoid development. It functions in the maturation of the 75-kD component of the translocon at the outer envelope membrane of chloroplasts and oxygen evolving complex subunit 33 (OE33) and involved in the maturation of PsbO, plastocyanin and FtsH2/8.
抗原信息 Recombinant part protein of Arabidopsis AT3G24590, Q8H0W1
同源基因 AT3G24590, LOC_Os02g16709等
验证应用 Western Blot
预测物种 油菜、辣椒、番茄、大豆、玉米、小麦等
保存条件 50% glycerol,-20 °C
理论/ 实际分子量 32.5 / 25 kDa for Arabidopsis and Rice
检测图片  
 
上样:约2 μg拟南芥和水稻叶片的总蛋白
电泳:12.5% SDS-PAGE
转膜:NC (nitrocellulose) 膜,电压10v过夜
封闭:5% 脱脂奶粉,室温孵育1h
一抗: 1:5000 稀释,室温孵育1h