莽草酸途径
Anti-SK2 / Shikimate kinase 2 antibody
- 货 号： PAB11004
- 基 因： AT4G39540, LOC_Os04g54800
- 别 名： Shikimate kinase
- 验证物种： 拟南芥、水稻
- 推荐稀释比： WB 1:2500---1:5000
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背 景 |
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Shikimate kinase catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate. |
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免疫原 |
Recombinant protein of Arabidopsis thaliana AT4G39540. |
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同源基因 |
AT2G21940， LOC_Os04g54800, Os04g0640600, Zm00001d002128, GRMZM2G004590, GLYMA08G14980, etc. |
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别 名 |
Shikimate kinase |
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验证应用 |
Western Blot |
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物种反应性 |
The sequence of the recombinant protein used for immunization is homologues with the sequence in Arabidopsis thaliana, rice, maize, soybean, rape, pepper, barley, etc. For more species homologues information, please contact tech support at TEL 400-880-3241 |
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保存 |
50% glycerol, |
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推荐稀释比 |
Western Blot (1:5000) |
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理论 / 实际分子量 |
35 / 30 kDa for Arabidopsis thaliana 31 / 29 kDa for Rice |
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检测图片 |
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上样： 1ug拟南芥/水稻叶片总蛋白 电泳：12.5% SDSPAGE 转膜： NC (nitrocellulose) 膜， 电压10 v过夜 封闭：5% 脱脂奶粉，室温孵育1h 一抗： 1:5000 稀释，室温孵育1h
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