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Anti-APX1/ L-ascorbate peroxidase 1, cytosolic

Anti-APX1/ L-ascorbate peroxidase 1, cytosolic

    货 号:PAB221203
    描 述:兔多克隆抗体
    基 因:AT1G07890,LOC_Os07g49400
    别 名:APX1, ASCORBATE PEROXIDASE 1, ATAPX01, ATAPX1, CS1, MATERNAL EFFECT EMBRYO ARREST 6, MEE6
    验证物种:拟南芥
    推荐稀释比:WB 1:2500---1:5000
 
背 景
 Ascorbate peroxidases are enzymes that scavenge hydrogen peroxide in plant cells. Eight types of APX have been described for Arabidopsis: three cytosolic (APX1, APX2, APX6), two chloroplastic types (stromal sAPX, thylakoid tAPX), and three microsomal (APX3, APX4, APX5) isoforms. At least part of the induction of heat shock proteins during light stress in Arabidopsis is mediated by H2O2 that is scavenged by APX1.
抗原信息 Recombinant part protein of Arabidopsis AT1G07890,Q05431
同源基因 AT1G07890,Os07g0694700
验证应用 Western Blot
预测物种 For more species homologues information, please contact tech support at TEL 400-880-3241
保存条件 50% glycerol,-20 °C
理论/ 实际分子量 27.6 / 26 kDa for Arabidopsis
检测图片  
   
 
 
 
上样:梯度稀释的抗原蛋白
           约3 μg拟南芥和水稻叶片的总蛋白    
电泳:10% SDS-PAGE
转膜:NC (nitrocellulose) 膜,电压10v过夜
封闭:5% 脱脂奶粉,室温孵育1h
一抗: 1:5000 稀释,室温孵育1h