三羧酸循环
Anti- MDH / Malate Dehydrogenase rabbit antibody
- 货 号： PAB210603
- 基 因： At3g47520, LOC_Os08g33720
- 别 名： MDH, Malate Dehydrogenase
- 验证物种： 拟南芥, 水稻
- 推荐稀释比： WB 1:2500---1:5000
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背 景 |
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Catalyzes a reversible NAD-dependent dehydrogenase reaction involved in central metabolism and redox homeostasis between organelle compartments. Plays a key role in the metabolism of dark chloroplasts and non-green plastids. Essential for embryo viability (PubMed:24198233, PubMed:24453164). Plays an essential role in heterotrophic metabolism in embryos, and autotrophic metabolism in photosynthetic tissues as well (PubMed:24453164). |
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免疫原 |
Recombinant protein of Arabidopsis thaliana At3g47520. |
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同源基因 |
At3g47520, LOC_Os08g33720, Zm00001d050409, GLYMA05G01010. |
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别 名 |
Malate Dehydrogenase, MDH, Plastidic NAD-Dependent Malate Dehydrogenase, PNAD-MDH |
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验证应用 |
Western Blot |
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物种反应性 |
For more species homologues information, please contact tech support at TEL 400-880-3241 |
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保存 |
50% glycerol, |
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推荐稀释比 |
Western Blot (1:5000) |
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理论 / 实际分子量 |
42 / 33 kDa for Arabidopsis thaliana |
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检测图片 |
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上样： 1ug拟南芥/水稻叶片总蛋白 电泳：12.5% SDS-PAGE 转膜： NC (nitrocellulose) 膜， 电压10 v过夜 封闭：5% 脱脂奶粉，室温孵育1h 一抗： 1:5000 稀释，室温孵育1h
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