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Anti-ACO3 / Aconitate hydratase 3 Rabbit Antibody

Anti-ACO3 / Aconitate hydratase 3  Rabbit Antibody

    货 号: PAB09052
    基 因: AT2G05710
    别 名: ACO1, ACO2, ACO3, Aconitase, Aconitate hydratase
    验证物种: 拟南芥, 水稻
    推荐稀释比: WB 1:2500---1:5000

 

背 景

Aconitase (ACO3) catalyzes the conversion of citrate to isocitrate through a cis-aconitate intermediate, indicating that it may participate in the TCA cycle and other primary metabolic pathways. The protein is believed to accumulate in the mitochondria and the cytosol.

抗原信息

Recombinant protein of Arabidopsis thaliana AT2G05710

同源基因

AT2G05710, AT4G26970, AT4G35830, LOC_Os08g09200, LOC_Os03g04410, LOC_Os06g19960

验证应用

Western Blot

物种反应性

油菜、辣椒、番茄、大豆、土豆、玉米、小麦、高粱、等

保存条件

50% glycerol-20 °C

理论/ 实际分子量

108 / 100 kDa for Arabidopsis thaliana and Rice

检测图片

 

 

上样:约2μg拟南芥和水稻叶片的总蛋白

电泳:12.5% SDS-PAGE

转膜:NC (nitrocellulose) 膜,电压10v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h