三羧酸循环

Anti-IDH1 / Isocitric dehydrogenase 1

Anti-IDH1 / Isocitric dehydrogenase 1

    货 号: PAB211211
    描 述:兔多克隆抗体
    基 因: At4g35260
    别 名:IDH-I, Isocitrate dehydrogenase [NAD] regulatory subunit 1, NAD(+)-specific ICDH 1
    验证物种:拟南芥
    推荐稀释比:WB 1:2500---1:5000
 

 

背 景

IDH1, located in the mitochondrion, plays an important role in the oxidative function of the citric acid cycle.

抗原信息

Recombinant protein of Arabidopsis thaliana At4g35260

同源基因

AT2G17130AT4G35650.

验证应用

Western Blot

预测物种

油菜、等

保存条件

50% glycerol-20 °C

理论/ 实际分子量

40 / 38 kDa for Arabidopsis thaliana

检测图片

 

 

 

上样:约2μg拟南芥和水稻叶片的总蛋白

电泳:12.5% SDS-PAGE

转膜:NC (nitrocellulose) 膜,电压10v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h