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SCS-beta/Succinate--CoA ligase [ADP-forming] subunit beta

SCS-beta/Succinate--CoA ligase [ADP-forming] subunit beta

    货 号:PAB220314
    描 述:兔多克隆抗体
    基 因:AT2G20420
    别 名:Succinyl-CoA synthetase beta chain, SCS-beta
    验证物种:拟南芥,水稻
    推荐稀释比:WB 1:2500---1:5000
 
背 景
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
免疫原 Recombinant protein of Arabidopsis thaliana AT2G20420
同源基因
AT2G20420, Os02g0621700
验证应用 Western Blot
物种反应性 For more species homologues information, please contact tech support at TEL 400-880-3241
保存条件 50% glycerol,-20 °C
理论/ 实际分子量 45 / 42 kDa for Arabidopsis thaliana and Rice
检测图片  
 
上样:约2μg拟南芥和水稻叶片的总蛋白
电泳:12.5% SDS-PAGE
转膜:NC (nitrocellulose) 膜,电压10v过夜
封闭:5% 脱脂奶粉,室温孵育1h
一抗: 1:5000 稀释,室温孵育1h