三羧酸循环
SCS-beta/Succinate--CoA ligase [ADP-forming] subunit beta
- 货 号:PAB220314
- 描 述:兔多克隆抗体
- 基 因:AT2G20420
- 别 名:Succinyl-CoA synthetase beta chain, SCS-beta
- 验证物种:拟南芥,水稻
- 推荐稀释比:WB 1:2500---1:5000
背 景 Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit. |
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免疫原 | Recombinant protein of Arabidopsis thaliana AT2G20420 |
同源基因 |
AT2G20420, Os02g0621700 |
验证应用 | Western Blot |
物种反应性 | For more species homologues information, please contact tech support at TEL 400-880-3241 |
保存条件 | 50% glycerol,-20 °C |
理论/ 实际分子量 | 45 / 42 kDa for Arabidopsis thaliana and Rice |
检测图片 | |
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上样:约2μg拟南芥和水稻叶片的总蛋白 电泳:12.5% SDS-PAGE 转膜:NC (nitrocellulose) 膜,电压10v过夜 封闭:5% 脱脂奶粉,室温孵育1h 一抗: 1:5000 稀释,室温孵育1h |