信号传递

Anti-MEKK1/Mitogen-activated protein kinase kinase kinase 1

Anti-MEKK1/Mitogen-activated protein kinase kinase kinase 1

    货 号:PAB21004
    描 述:兔多克隆抗体
    基 因:At4g08500
    别 名:ARAKIN, AtMEKK1, MAP kinase kinase kinase 1, MAPKKK8
    验证物种:拟南芥,水稻
    推荐稀释比:WB 1:2500---1:5000
                    
背 景
The MEKK (MAPK/ERK kinase kinase) family has four members: At4g08500 (MEKK1), At4g08480 (MEKK2), At4g08470 (MEKK3) and At4g12020 (MEKK4). They function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses, and also play an important role in pathogen defense by negatively regulating innate immunity.
免疫原 Recombinant protein of Arabidopsis thaliana At4g08500
同源基因 At4g08500
验证应用 Western Blot
物种反应性 For more species homologues information, please contact tech support at TEL 400-880-3241
保存条件 50% glycerol,-20 °C
理论/ 实际分子量 66 kDa for Arabidopsis thaliana
检测图片  
 
上样:约2μg拟南芥和水稻叶片的总蛋白
电泳:12.5% SDS-PAGE
转膜:NC (nitrocellulose) 膜,电压10v过夜
封闭:5% 脱脂奶粉,室温孵育1h
一抗:1:5000 稀释,室温孵育1h