信号传递

Anti-OXI1/ Serine/threonine-protein kinase antibody

Anti-OXI1/  Serine/threonine-protein kinase antibody

    货 号: PAB210417
    描 述:兔多克隆抗体
    基 因: At3g25250
    别 名:AGC2, AGC2 KINASE 1, AGC2-1, ATOXI1, OXIDATIVE SIGNAL-INDUCIBLE1
    验证物种:拟南芥
    推荐稀释比:WB 1:2500---1:5000
 
背 景
OXI1 is involved in oxidative burst-mediated signaling. It is partly required for the activation of MPK3 and MPK6 by hydrogen peroxide and cellulase elicitor.
抗原信息 Recombinant protein of Arabidopsis thaliana  At3g25250
同源基因 At3g25250
验证应用 Western Blot
预测物种 油菜
保存条件 50% glycerol,-20 °C
理论/ 实际分子量 47.6 kDa for Arabidopsis thaliana
检测图片  
 
上样:约2μg拟南芥和水稻叶片的总蛋白
电泳:12.5% SDS-PAGE
转膜:NC (nitrocellulose) 膜,电压10v过夜
封闭:5% 脱脂奶粉,室温孵育1h
一抗: 1:5000 稀释,室温孵育1h