信号传递

Anti-GLB1/ Nitrogen regulatory protein P-II homolog

Anti-GLB1/ Nitrogen regulatory protein P-II homolog

    货 号:PAB220206
    描 述:兔多克隆抗体
    基 因:AT4G01900
    别 名:GLB1, GLNB1 HOMOLOG, PII
    验证物种:油菜,水稻
    推荐稀释比:WB 1:2500---1:5000
 
背 景
GLB1 encodes a PII protein that may function as part of a signal transduction network involved in perceiving the status of carbon and organic nitrogen. It forms a protein complex with N-acetylglutamate kinase and regulates the kinase activity by relieving the feedback inhibition of the kinase by arginine.
抗原信息 Recombinant part protein of Arabidopsis thaliana AT4G01900, Q9ZST4
同源基因
AT4G01900,CDX91831
验证应用 Western Blot
预测物种 拟南芥、辣椒、番茄、大豆、小麦、高粱
保存条件 50% glycerol,-20 °C
理论/ 实际分子量 21 / 15 kDa for Brassica napus L. and Rice
检测图片  
 
上样:约2μg油菜和水稻叶片的总蛋白
电泳:12.5% SDS-PAGE
转膜:NC (nitrocellulose) 膜,电压10v过夜
封闭:5% 脱脂奶粉,室温孵育1h
一抗: 1:5000 稀释,室温孵育1h