氧化磷酸化

Anti-VHA-A/V-type proton ATPase catalytic subunit A

Anti-VHA-A/V-type proton ATPase catalytic subunit A

    货 号: PAB211231
    描 述:兔多克隆抗体
    基 因: AT1G78900
    别 名:V-ATPase subunit A
    验证物种:拟南芥,水稻
    推荐稀释比:WB 1:2500---1:5000

 

背 景

The catalytic subunit A of the vacuolar ATP synthase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

抗原信息

Recombinant protein of Arabidopsis thaliana AT1G78900

同源基因

AT1G78900, LOC_Os06g45120, LOC_Os02g07870

验证应用

Western Blot

预测物种

油菜、辣椒、番茄、大豆、土豆、玉米、小麦、高粱、等

保存条件

50% glycerol-20 °C

理论/ 实际分子量

69 kDa for Arabidopsis thaliana and Rice

检测图片

 

 

上样:约2μg拟南芥和水稻叶片的总蛋白

电泳:12.5% SDS-PAGE

转膜:NC (nitrocellulose) 膜,电压10v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h