叶绿素合成

Anti-ERS / Glutamate--tRNA ligase rabbit antibody

Anti-ERS / Glutamate--tRNA ligase rabbit antibody

    货 号: PAB09001
    描 述:兔多克隆抗体
    基 因: At5g64050, Os02g0121000
    别 名:GluRS, Glutamyl-tRNA synthetase, Protein OVULE ABORTION 3, OVA3, ERS
    验证物种:拟南芥,水稻
    推荐稀释比:WB 1:2500---1:5000
 
背 景
AtERS catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).
免疫原 Recombinant protein of Arabidopsis thaliana At5g64050
同源基因 At5g64050, Os02g0121000
验证应用 Western Blot
物种反应性 For more species homologues information, please contact tech support at TEL 400-880-3241
保存条件 50% glycerol,-20 °C
理论/ 实际分子量 63.5/52 kDa for Arabidopsis thaliana
62.9/52 kDa for Rice
检测图片  
 
上样:约2μg拟南芥和水稻叶片的总蛋白
电泳:12.5% SDS-PAGE
转膜:NC (nitrocellulose) 膜,电压10v过夜
封闭:5% 脱脂奶粉,室温孵育1h
一抗: 1:5000 稀释,室温孵育1h