叶绿素合成
Anti-GUN4/ Genomes uncoupled 4
- 货 号：PAB220419
- 描 述：兔多克隆抗体
- 基 因：AT3G59400
- 别 名：Tetrapyrrole-binding protein, GENOMES UNCOUPLED 4
- 验证物种：拟南芥
- 推荐稀释比：WB 1:2500---1:5000
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背 景 GUN, genomes uncoupled, is necessary for coupling the expression of some nuclear genes to the functional state of the chloroplast. GUN4 regulates chlorophyll synthesis and plastid-to-nucleus signal transduction by binding both the product and the substrate of Mg-chelatase, an enzyme that produces magnesium-protoporphyrin IX (Mg-Proto). |
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| 抗原信息 | Recombinant part protein of Arabidopsis AT3G59400 |
| 同源基因 | AT3G59400 |
| 验证应用 | Western Blot |
| 预测物种 | 油菜 |
| 保存条件 | 50% glycerol，-20 °C |
| 理论/ 实际分子量 | 29.7 / 22 kDa for Arabidopsis |
| 检测图片 | |
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上样：约2 μg拟南芥和水稻叶片的总蛋白 电泳：12.5% SDS-PAGE 转膜：NC (nitrocellulose) 膜，电压10v过夜 封闭：5% 脱脂奶粉，室温孵育1h 一抗： 1:5000 稀释，室温孵育1h |
