转录因子
Anti-GRF /G-BOX regulating factor rabbit antibody
- 货 号： PAB25003
- 基 因： AT5G10450, LOC_Os08g33370, etc.
- 别 名： G-BOX REGULATING FACTOR, General regulatory element
- 验证物种： 拟南芥、水稻
- 推荐稀释比： WB 1:2500---1:5000
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背 景 |
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Encodes a member of the 14-3-3 gene family that is a lambda isoform (14-3-3λ). Interacts with APX3 (ascorbate peroxidase) and AKR2, suggesting a role in mediating oxidative metabolism in stress response. This protein was shown to colocalize and interact with SERK1 by which it is phosphorylated. This protein is also reported to interact with the phosphorylated form of the BZR1 transcription factor involved in brassinosteroid signaling and may affect the nucleocytoplasmic shuttling of BZR1. Interacts with JAZ10.4 which lacks the Jas motif. It is also phosphorylated by CRPK1 as part of the response to cold and translocate to the nucleus after phosphorylation. |
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免疫原 |
Recombinant protein of Arabidopsis thaliana AT5G10450. |
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同源基因 |
AT5G10450, AT4G09000, AT1G22300, AT1G34760, AT1G26480, AT1G78300, AT5G38480, AT5G16050, AT3G02520, AT5G65430, AT2G42590, AT1G35160, LOC_Os08g33370, etc. |
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别 名 |
14-3-3 protein G-BOX FACTOR14 LAMBDA, 14-3-3LAMBDA, AFT1, G-BOX regulating factor 6, GRF6 |
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验证应用 |
Western Blot |
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物种反应性 |
For more species homologues information, please contact tech support at TEL 400-880-3241 |
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保存 |
50% glycerol, |
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推荐稀释比 |
Western Blot (1:5000) |
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理论 / 实际分子量 |
27 / 27 kDa for Arabidopsis thaliana |
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检测图片 |
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上样： 1ug拟南芥/水稻叶片总蛋白 电泳：12.5% SDS-PAGE 转膜： NC (nitrocellulose) 膜， 电压10 v过夜 封闭：5% 脱脂奶粉，室温孵育1h 一抗： 1:5000 稀释，室温孵育1h
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