光系统I

Anti-PsaF/ PSI-F subunit of photosystem I rabbit antibody

Anti-PsaF/ PSI-F subunit of photosystem I rabbit antibody

    货 号: PAB01006
    描 述:兔多克隆抗体
    基 因: At1g31330
    别 名:PSAF, PSI-F, PHOTOSYSTEM I SUBUNIT F, Light-harvesting complex I 17 kDa protein
    验证物种:拟南芥,水稻
    推荐稀释比:WB 1:2500---1:5000
 

 

背 景

PsaF participates in efficiency of electron transfer from plastocyanin to P700 (or cytochrome c553 in algae and cyanobacteria). This plastocyanin-docking protein contributes to the specific association of plastocyanin to PSI.

抗原信息

BSA-conjugated synthetic peptide derived from Arabidopsis thaliana At1g31330

同源基因

At1g31330, LOC_Os03g56670

验证应用

Western Blot

预测物种

油菜、辣椒、番茄、大豆、土豆、玉米、小麦、高粱、等

保存条件

50% glycerol-20 °C

理论/ 实际分子量

24/17 kDa for Arabidopsis thaliana

检测图片

 

 

 

上样:约2μg拟南芥和水稻叶片的总蛋白

电泳:12.5% SDS-PAGE

转膜:NC (nitrocellulose) 膜,电压10v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h

上样:约2μg油菜和水稻叶片的总蛋白

电泳:15% Tricine-SDS-PAGE

转膜:NC (nitrocellulose) 膜,电压10v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h