F-型 ATP合酶复合体
Anti-ATPF / ATP synthase subunit b rabbit antibody
- 货 号： PAB04006
- 基 因： ATCG00130, LOC_Os10g38272
- 别 名： AtpF, ATP synthase F(0) sector subunit b, CF0I.
- 验证物种： 拟南芥、水稻
- 推荐稀释比： WB 1:2500---1:10000
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背 景 |
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F1F0 ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F1 containing the extramembraneous catalytic core and F0 containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Component of the F0 channel, it forms part of the peripheral stalk, linking F1 to F0. |
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免疫原 |
Recombinant protein of Arabidopsis thaliana ATCG00130. |
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基 因 |
LOC_Os10g38272, ZemaCp019, GLYMA12G36098, etc. |
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别 名 |
AtpF, ATP synthase F(0) sector subunit b, CF0I. |
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验证应用 |
Western Blot |
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物种反应性 |
For more species homologues information, please contact tech support at TEL 400-880-3241 |
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保存 |
50% glycerol, |
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推荐稀释比 |
Western Blot (1:2500-10000) |
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理论 / 实际 分子量 |
21 / 16 kDa for Arabidopsis thaliana 21 / 17 kDa for rice |
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检测图片 |
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上样：2ug拟南芥/水稻叶片总蛋白 电泳：15 % SDS-PAGE 转膜： NC (nitrocellulose) 膜， 电压10 v过夜 封闭：5% 脱脂奶粉，室温孵育1h 一抗： 1:5000 稀释，室温孵育1h
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