F-型 ATP合酶复合体

Anti-ATPG / PDE334 / ATP synthase beta chain (Subunit II)

Anti-ATPG / PDE334 / ATP synthase beta chain (Subunit II)

    货 号: PAB04007
    基 因: AT4G32260, LOC_Os03g17070
    别 名: AtpG, ATPase subunit II, ATP synthase F(0) sector subunit b'.
    验证物种: 拟南芥、水稻
    推荐稀释比: WB 1:2500---1:10000
 

背 景

ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.

免疫原

Recombinant protein of Arabidopsis thaliana AT4G32260.

同源基因

LOC_Os03g17070, Zm00001d047789, GLYMA04G07010. etc.

别 名

AtpG, ATPase subunit II, ATP synthase F(0) sector subunit b'.

验证应用

Western Blot

物种反应性

For more species homologues information, please contact tech support at TEL 400-880-3241

保存

50% glycerol

推荐稀释比

Western Blot (1: 2500-10000)

理论 / 实际分子量

24 / 16 kDa for Arabidopsis thaliana
23 / 15 kDa for rice

检测图片

 

上样:2ug拟南芥/水稻叶片总蛋白
电泳:15 % SDS-PAGE
转膜: NC (nitrocellulose) 膜, 电压10 v过夜
封闭:5% 脱脂奶粉,室温孵育1h
一抗: 1:5000 稀释,室温孵育1h