Calvin 循环

Anti-PRK / Phosphoribulokinase rabbit antibody

Anti-PRK / Phosphoribulokinase rabbit antibody

    货 号:PAB07007
    描 述:兔多克隆抗体
    基 因:AT1G32060
    别 名:Phosphoribulokinase, PRK
    验证物种:拟南芥,水稻
    推荐稀释比:WB 1:2500---1:5000

 

背 景

Phosphoribulokinase is an enzyme that catalyzes ATP and D-ribulose 5-phosphate to produce ADP and D-ribulose 1,5-bisphosphate. This protein is involved in the pathway Calvin cycle.

抗原信息

Recombinant protein of Arabidopsis thaliana AT1G32060

同源基因

AT1G32060, LOC_Os02g47020LOC_Os04g50880

验证应用

Western Blot

物种反应性

油菜、辣椒、番茄、大豆、土豆、玉米、小麦、高粱、等

保存条件

50% glycerol-20 °C

理论/ 实际分子量

44.5 / 45 kDa for Arabidopsis thaliana

44.9 / 45 kDa for Rice

检测图片

 

 

上样:约2μg拟南芥和水稻叶片的总蛋白

电泳:12.5% SDS-PAGE

转膜:NC (nitrocellulose) 膜,电压10v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h