Calvin 循环

Anti-RCA / Rubisco activase rabbit antibody

Anti-RCA / Rubisco activase rabbit antibody

    货 号: PAB07003
    基 因: AT2G39730, LOC_Os11g47970.
    别 名: RCA, Ribulose bisphosphate carboxylase/oxygenase activase
    验证物种: 拟南芥、水稻
    推荐稀释比: WB 1:2500---1:5000

 

背 景

There are two forms of activase (alpha and beta) in some species (for example Arabidopsis, Camelina, Spinach, Rice) and only one form in other species (Tobacco, Maize, Chlamydomonas). Alpha is about 46-47 kDa, beta is about 42 kDa. Species that have only one form have the beta form.

免疫原

Recombinant protein of Arabidopsis thaliana AT2G39730.

同源基因

AT2G39730, LOC_Os11g47970, Zm00001d048592, GLYMA02G41700

验证应用

Western Blot

物种反应性

For more species homologues information, please contact tech support at TEL 400-880-3241

保存

50% glycerol, -20 °C

推荐稀释比

Western Blot (1:5000)

理论 / 实际分子量

52 / 45 kDa for Arabidopsis thaliana and Rice

检测图片

 

 

 

 

上样: 2 μg拟南芥/水稻叶片总蛋白

电泳:12.5% SDS-PAGE

转膜: NC (nitrocellulose) 膜, 电压10 v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h