Calvin 循环

Anti-RbcL / Ribulose bisphosphate carboxylase large subunit

Anti-RbcL / Ribulose bisphosphate carboxylase large subunit

    货 号: PAB07001
    描 述:兔多克隆抗体
    基 因: ATCG00490, LOC_Os10g21268
    别 名:RbcL, RuBisCO large subunit
    验证物种:拟南芥,水稻
    推荐稀释比:WB 1:2500---1:5000
 
背 景
Ribulose-1,5-bisphosphate carboxylase/oxygenase commonly known by the abbreviation RuBisCO. RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. The enzyme usually consists of two types of protein subunit, called the large chain (RBCL) and the small chain (RBCS).
免疫原 Recombinant protein of Arabidopsis thaliana ATCG00490
同源基因 LOC_Os10g21268, Zm00001d000279, GLYMA12G06606. etc.
验证应用 Western Blot
物种反应性 For more species homologues information, please contact tech support at TEL 400-880-3241
保存条件 50% glycerol,-20 °C
理论/ 实际分子量 53 / 53 kDa for Arabidopsis thaliana
53 / 53 kDa for Rice
检测图片  
 
上样:约2μg拟南芥和水稻叶片的总蛋白
电泳:12.5% SDS-PAGE
转膜:NC (nitrocellulose) 膜,电压10v过夜
封闭:5% 脱脂奶粉,室温孵育1h
一抗:1:5000 稀释,室温孵育1h