光呼吸
Anti-GGAT / Glutamate-glyoxylate aminotransferase
- 货 号： PAB07033
- 基 因： AT1G23310, LOC_Os07g01760
- 别 名： glutamate:glyoxylate aminotransferase, AOAT, GGAT, GGT
- 验证物种： 拟南芥、水稻
- 推荐稀释比： WB 1:2000---1:5000
|
|
|
|
背 景 |
|
|
GGAT catalyzes the glutamate:glyoxylate (GGT or GGAT), alanine:glyoxylate (AGT), alanine:2-oxoglutarate (AKT) and glutamate:pyruvate (GPT) aminotransferase reactions in peroxisomes. It is required for abscisic acid (ABA)- and stress-mediated responses in an H2O2-dependent manner. It functions as a photorespiratory aminotransferase that modulates amino acid content during photorespiration (GGAT activity); promotes serine, glycine and citrulline metabolism in response to light. |
|
|
免疫原 |
Recombinant protein of Arabidopsis thaliana AT1G23310. |
|
同源基因 |
AT1G70580, LOC_Os07g01760, Os07g0108300, Zm00001d018639, GRMZM2G120563, GLYMA02G04320, etc. |
|
别 名 |
alanine-2-oxoglutarate aminotransferase, glutamate:glyoxylate aminotransferase, glutamate:glyoxylate aminotransferase, AOAT, GGAT, GGT |
|
验证应用 |
Western Blot |
|
物种反应性 |
For more species homologues information, please contact tech support at TEL 400-880-3241 |
|
保存 |
50% glycerol, |
|
推荐稀释比 |
Western Blot (1:5000) |
|
理论 / 实际分子量 |
53.3 / 49 kDa for Arabidopsis thaliana 53.7 / 49 kDa for Rice |
|
检测图片 |
|
|
|
上样： 1ug拟南芥/水稻叶片总蛋白 电泳：12.5% SDSPAGE 转膜： NC (nitrocellulose) 膜， 电压10 v过夜 封闭：5% 脱脂奶粉，室温孵育1h 一抗： 1:5000 稀释，室温孵育1h
|
