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Anti-DIT1 / Dicarboxylate transporter 1

Anti-DIT1 / Dicarboxylate transporter 1

    货 号: PAB07044
    基 因: AT5G12860, LOC_Os12g33080
    别 名: Dicarboxylate transporter 1, DIT1
    验证物种: 拟南芥、水稻
    推荐稀释比: WB 1:2500---1:5000
    亚细胞定位:chloroplast inner membrane

 

背 景

DIT1 is a 2-oxoglutarate/malate translocator involved with DIT2-1 in primary ammonia assimilation and in the re-assimilation of ammonia generated by the photorespiratory pathway. Imports 2-oxoglutarate into plastids as precursor for ammonia assimilation. 2-oxoglutarate is converted to glutamate, the end product of ammonia assimilation, which is exported to the cytosol by DIT2-1.

免疫原

Recombinant protein of Arabidopsis thaliana AT5G12860.

同源基因

LOC_Os12g33080, Os12g0515400, Zm00001d023929, GRMZM2G383088, GLYMA12G06190, etc.

验证应用

Western Blot

物种反应性

For more species homologues information, please contact tech support at TEL 400-880-3241

保存

50% glycerol, -20 °C

理论 / 实际分子量

59 / 50 kDa for Arabidopsis thaliana

58 / 52 kDa for  Rice

检测图片

 

 

上样:μg拟南芥/水稻叶片总蛋白

电泳:12.5% SDSPAGE

转膜: NC (nitrocellulose) 膜, 电压10 v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h