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Anti-SHM1 / Serine hydroxymethyltransferase 1

Anti-SHM1 / Serine hydroxymethyltransferase 1

    货 号: PAB07039
    基 因: AT4G37930, LOC_Os03g52840
    别 名: SHM1,SHMT1,STM,Serine hydroxymethyltransferase
    验证物种: 拟南芥、水稻
    推荐稀释比: WB 1:2500---1:5000

 

背 景

Serine hydroxymethyltransferases (SHMs) are important enzymes of cellular one-carbon metabolism and are essential for the photorespiratory glycine-into-serine conversion in leaf mesophyll mitochondria. SHM1 catalyzes the conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate.

免疫原

Recombinant protein of Arabidopsis thaliana AT4G37930.

同源基因

LOC_Os03g52840, Zm00001d030859, GLYMA18G27710. etc.

别 名

SHM1, Serine hydroxymethyltransferase 1, serine transhydroxymethyltransferase, serine transhydroxymethyltransferase 1, SHMT1, STM.

验证应用

Western Blot

物种反应性

For more species homologues information, please contact tech support at TEL 400-880-3241

保存

50% glycerol

推荐稀释比

Western Blot (1:5000-1:10000)

理论 / 实际分子量

52 / 57 kDa for Arabidopsis thaliana

52 / 56 kDa for rice

检测图片

 

上样:2ug拟南芥/水稻叶片总蛋白

电泳:12.5% SDS-PAGE

转膜: NC (nitrocellulose) 膜, 电压10 v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h