氧化还原

Anti-CBR1/ NADH--cytochrome b5 reductase 1

Anti-CBR1/ NADH--cytochrome b5 reductase 1

    货 号:PAB220449
    描 述:兔多克隆抗体
    基 因:AT5G17770
    别 名:ATCBR, CBR, CBR1, NADH:CYTOCHROME B5 REDUCTASE 1
    验证物种:油菜, 水稻
    推荐稀释比:WB 1:2500---1:5000
 
背 景
CBR1 encodes NADH:cytochrome (Cyt) b5 reductase that displayed strict specificity to NADH for the reduction of a recombinant Cyt b5 (AtB5-A), whereas no Cyt b5 reduction was observed when NADPH was used as the electron donor.
抗原信息 Recombinant part protein of Arabidopsis thaliana AT5G17770,Q9ZNT1
同源基因
AT5G17770, CDX78759, LOC_Os05g40990
验证应用 Western Blot
预测物种 拟南芥、辣椒、番茄、大豆、玉米、小麦、高粱等
保存条件 50% glycerol,-20 °C
理论/ 实际分子量 31.5 / 27 kDa for Brassica napus L. and Rice
检测图片  
 
上样:约2μg油菜和水稻叶片的总蛋白
电泳:12.5% SDS-PAGE
转膜:NC (nitrocellulose) 膜,电压10v过夜
封闭:5% 脱脂奶粉,室温孵育1h
一抗: 1:5000 稀释,室温孵育1h