内参抗体

Anti-RBCL / Ribulose bisphosphate carboxylase large subunit

Anti-RBCL / Ribulose bisphosphate carboxylase large subunit

    货 号: PAB07001
    基 因: ATCG00490,LOC_Os10g21268
    别 名: RBCL, Ribulose Bisphosphate Carboxylase large subunit
    验证物种: 拟南芥,水稻
    推荐稀释比: WB 1:2500---1:10000

 

背 景

Ribulose-1,5-bisphosphate carboxylase/oxygenase commonly known by the abbreviation RuBisCO, RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. The enzyme usually consists of two types of protein subunit, called the large chain (RBCL) and the small chain (RBCS).

免疫原

Recombinant protein of Arabidopsis thaliana ATCG00490.

同源基因

LOC_Os10g21268, Zm00001d000279, GLYMA12G06606. etc.

别 名

RBCL, Ribulose Bisphosphate Carboxylase large subunit.

验证应用

Western Blot

物种反应性

For more species homologues information, please contact tech support at TEL 400-880-3241

保存

50% glycerol,-20 °C

理论 / 实际分子量

53 / 53-55 kDa for Arabidopsis thaliana

53 / 53 kDa for rice

检测图片

 

上样:2 ug拟南芥/水稻叶片总蛋白

电泳:12.5% SDS-PAGE

转膜: NC (nitrocellulose) 膜, 电压10 v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h