内参抗体

Anti-GAPDH/ Glyceraldehyde-3-phosphate dehydrogenase GAPC2

Anti-GAPDH/ Glyceraldehyde-3-phosphate dehydrogenase GAPC2

    货 号: PAB002C
    描 述:兔多克隆抗体
    基 因: At1g13440, LOC_Os04g40950
    别 名:GAPC-2, GAPC2, NAD-dependent glyceraldehydephosphate dehydrogenase C subunit 2
    验证物种:拟南芥,水稻
    推荐稀释比:WB 1:2500---1:5000

 

背 景

GAPDH is a key enzyme in glycolysis (catalyzing D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate). Involved in the maintenance of cellular ATP levels and carbohydrate metabolism.

免疫原

BSA-conjugated synthetic peptide derived from Arabidopsis thaliana At1g13440

同源基因

 At1g13440, LOC_Os04g40950

验证应用

Western Blot

物种反应性

For more species homologues information, please contact tech support at TEL 400-880-3241

保存条件

50% glycerol-20 °C

理论/ 实际分子量

37 kDa for Arabidopsis thaliana and Rice

检测图片

 

 

 

上样:约2μg拟南芥和水稻叶片的总蛋白

电泳:12.5% SDS-PAGE

转膜: NC (nitrocellulose) 膜,电压10v过夜

封闭:5% 脱脂奶粉,室温孵育1h

一抗: 1:5000 稀释,室温孵育1h