信号传递
Anti-GID1 / Gibberellin receptor GID1 antibody
- 货 号： PAB01201
- 基 因： LOC_Os05g33730
- 别 名： gibberellin receptor GID1L2
- 验证物种： 水稻
- 推荐稀释比： WB 1:2500---1:10000
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背 景 |
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GID1 functions as soluble gibberellin (GA) receptor. GA is an essential hormone that regulates growth and development in plants. Binds with high affinity the biologically active GAs such as GA1, GA3 and GA4, but has low or no affinity for the biologically inactive GAs. Upon GA-binding, it interacts with the DELLA protein SLR1, a repressor of GA signaling. This leads to SLR1 degradation by the proteasome, allowing the GA signaling pathway. |
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免疫原 |
Recombinant protein of Rice LOC_Os05g33730. |
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其他物种同源基 因 |
AT5G27320, Zm00001d038165, GRMZM2G173630, GLYMA03G30460, etc. |
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验证应用 |
Western Blot |
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物种反应性 |
The sequence of the recombinant protein used for immunization is homologues with the sequence in Arabidopsis thaliana, rice, maize, soybean, rape, pepper, barley, etc. For more species homologues information, please contact tech support at TEL 400-880-3241 |
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保存 |
50% glycerol, |
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推荐稀释比 |
Western Blot (1:5000) |
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理论 / 实际 分子量 |
40 / 40 kDa for Rice |
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检测图片 |
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上样： 从左到右依次为0.5ug、1ug、2ug水稻叶片总蛋白 电泳：12.5% SDS-PAGE 转膜： NC (nitrocellulose) 膜， 电压10 v过夜 封闭：5% 脱脂奶粉，室温孵育1h 一抗： 1:5000 稀释，室温孵育1h
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