背 景

EIF3E is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.

抗原信息

Recombinant part protein of Arabidopsis thaliana AT3G57290，Q9C5Z3

同源基因

AT3G57290

验证应用

Western Blot (WB)，Immunoprecipitation (IP)

预测物种

For more species homologues information, please contact tech support at TEL 400-880-3241

保存条件

50% glycerol，-20 °C

理论/ 实际分子量

51.8 kDa for Arabidopsis thaliana

检测图片

上样：梯度稀释的抗原蛋白

电泳：12% SDS-PAGE

转膜：NC (nitrocellulose) 膜，电压10v过夜

封闭：5% 脱脂奶粉，室温孵育1h

一抗： 1:5000 稀释，室温孵育1h