背 景

HPR catalyzes the NADH-dependent reduction of hydroxypyruvate into glycerate in the photorespiratory core cycle. Mediates fatty acid beta-oxidation in germinating seeds when malate dehydrogenase is absent.

免疫原

Recombinant protein of Arabidopsis thaliana AT1G68010.

同源基因

LOC_Os02g01150, Os02g0101500, Zm00001d014919, GRMZM2G074282, GLYMA20G24200, etc.

别 名

HPR, Glycerate dehydrogenase HPR, Hydroxypyruvate reductase

验证应用

Western Blot

物种反应性

For more species homologues information, please contact tech support at TEL 400-880-3241

保存

50% glycerol,

推荐稀释比

Western Blot (1:5000)

理论 / 实际分子量

42 / 45 kDa for Arabidopsis thaliana

43 / 45 kDa for  Rice

检测图片

上样： 1ug拟南芥/水稻叶片总蛋白

电泳：12.5% SDSPAGE

转膜： NC (nitrocellulose) 膜， 电压10 v过夜

封闭：5% 脱脂奶粉，室温孵育1h

一抗： 1:5000 稀释，室温孵育1h